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Phospho-creb

WebRayBio® Phospho-CREB (S133) and Total CREB ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated CREB protein in your experimental model system, you can verify pathway activation in your cell lysates. ... WebPhospho-CREB (Ser133) Antibody (44-298G) in ICC/IF Immunofluorescent analysis of Phospho-CREB pSer133 Antibody was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature.

Identification of compounds that potentiate CREB signaling as ... - PNAS

WebApr 11, 2024 · The classical PKC/ERK/CREB signaling pathway is involved in the synthesis of pituitary TSH . To evaluate whether the PKC/ERK/CREB signaling pathway mediates the regulation of TSH with CK1α, we analyzed the phosphorylation levels of PKC, ERK, and CREB in the mouse pituitary gland after treatment with 1 mg/mL D4476 or 5 mg/mL pyrvinium. WebTime-dependent induction of CREB phosphorylation in the hippocampus by the endogenous cannabinoid Masako Isokawa Department of Biological Sciences, The University of Texas at Brownsville, Brownsville TX 78520 Abstract The involvement of the endogenous cannabinoid system has been implicated in the rewarding actions of several drugs of abuse. northeast vocational technology center https://thencne.org

Anti-phospho-CREB (Ser133) Antibody 06-519 - EMD Millipore

WebJun 28, 2024 · Here, we have investigated phosphorylation of the cAMP-responsive element binding protein (CREB) and activation of the immediate early gene c-Fos pathway following the induction of synaptic plasticity by theta-burst stimulation (TBS) in acute cerebellar slices. LTP and LTD were localized using voltage-sensitive dye imaging (VSDi). WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity … WebPhosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in ... northeast volleyball tournament

Phospho-CREB (Ser133) cellular HTRF kit Cisbio - PerkinElmer

Category:Phospho-CREB-1(Ser133)Polyclonal Antibody E-AB-20849

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Phospho-creb

p-CREB1 Antibody (10E9) SCBT - Santa Cruz Biotechnology

WebAnti-phospho-CREB (Ser133) Antibody, clone 10E9 is a Mouse Monoclonal Antibody for detection of phospho-CREB (Ser133) also known as active transcription factor CREB & has been validated in WB, ELISA. ELISA: Recommended at 0.05 μg/mL by an independent laboratory. Research Category Epigenetics & Nuclear Function Research Sub Category WebMar 29, 2024 · The CREB binding domain, referred to as KIX, is α helical and binds to an unstructured kinase-inducible domain in CREB following phosphorylation of CREB at Ser-133. Phospho-Ser-133 forms direct contacts with residues in KIX, and these contacts are further stabilized by hydrophobic residues in the kinase-inducible domain which flank …

Phospho-creb

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WebThis ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody & Primer Set (Antibody ChIP) conveniently includes the antibody & the specific control PCR primers. MSDS … WebCREB-TF (CREB, cAMP response element-binding protein) [1] is a cellular transcription factor. It binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the genes. [2] CREB was first described in 1987 as a cAMP -responsive transcription factor regulating the somatostatin gene. [3]

WebProduct name Anti-CREB (phospho S133) antibody See all CREB primary antibodies Description Rabbit polyclonal to CREB (phospho S133) Host species Rabbit Tested applications Suitable for: WB, ICC/IF more details … WebApr 9, 2024 · Phospho-S133 CREB works in combination with nuclear CRTC1/2 to induce expression of Prg4 in articular chondrocytes. While we observed that administration of FFSS of ∼7.6 dyn/cm 2 can induce the expression of Prg4 in either a subpopulation of murine epiphyseal chondrocytes or bovine superficial articular chondrocytes in vitro, it is unclear ...

WebMar 29, 2024 · The CREB binding domain, referred to as KIX, is α helical and binds to an unstructured kinase-inducible domain in CREB following phosphorylation of CREB at Ser … WebHuman Phospho-CREB (S133) Antibody Summary Species Reactivity Human Specificity Detects human CREB when phosphorylated at S133. Source Monoclonal Mouse IgG 2A Clone # 702710 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human CREB S133 site Formulation

WebMar 15, 2014 · CREB is phosphorylated on Ser133 by PKA (protein kinase A), promoting the recruitment of the co-activator proteins CBP (CREB-binding protein) and p300; this has …. …

WebSelected: P-CREB Antibody $ 1,795.00 $ 895.00 1,795.00 $ 895.00. P-CREB Antibody quantity northeast vokeWebPhospho-CREB (S133) 1-plate assay protocol Detection of Phosphorylated CREB (S133) with HTRF reagents can be performed in a single plate used for culturing, stimulation and … northeast vs centralWebFeb 17, 2009 · To test whether any CREB potentiating compounds identified in the screen acted on PKA, compounds were tested in a PKA enzyme based assay that measured phosphorylation of a target peptide. 8-bromo-cAMP and 8-(4-chlorophenylthio)-cAMP, which are cAMP analogues, were found to be active in the primary CRE-β-lactamase screen, both … northeast votech pryorWebアズワンの【AXEL】Elabscience Phospho-CREB-1(Ser133)Polyclonal Antibody E-AB-20849のコーナーです。AXELは研究開発、医療介護、生産現場、食品衛生など幅広い分野に750万点以上の品揃えでお応えする商品サイト。3000円以上ご注文で送料無料。 how to reverse send imali on nedbank appWebProduct name Anti-CREB (phospho S129 + S133) antibody See all CREB primary antibodies Description Rabbit polyclonal to CREB (phospho S129 + S133) Host species Rabbit Tested applications Suitable for: ICC/IF, WB more details Species reactivity Reacts with: Mouse, Human Immunogen Synthetic peptide corresponding to Human CREB (phospho S129 + … northeast voke wakefield maWebMar 21, 2024 · Stimulated by phosphorylation. Phosphorylation of both Ser-119 and Ser-128 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-119 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). northeast vs midwestWebMar 24, 2024 · p-CREB-1 Antibody (10E9) is an IgG1 κ mouse monoclonal p-CREB-1 antibody (also designated p-CREB-1 antibody or CREB antibody) suitable for the detection of Ser 133 phosphorylated CREB-1 and Ser 63 phosphorylated ATF-1 of mouse, rat and human origin by WB and IP. p-CREB-1 Antibody (10E9) is available as the non-conjugated anti-p … northeast vs mid atlantic