Nettet13. mai 2024 · Thus, in mice, the pseudostratified epithelium containing Trp63-Krt5 double-positive BSCs resides exclusively in the trachea and proximal main stem bronchi [10, 12, 13]. Moreover, in the murine proximal bronchiolar airways, the pseudostratified epithelium transitions into a simple cuboidal epithelium devoid of BSCs, predominantly … Nettet1. okt. 2004 · We characterized the tracheal and bronchial relaxation caused by proteinase-activated receptor-2 (PAR-2) activation in ddY mice and/or in wild-type and PAR-2-knockout mice of C57BL/6 background. Ser-Leu-Ile-Gly-Arg-Leu-amide (SLIGRL-NH2) and Thr-Phe-Leu-Leu-Arg-amide, PAR-2- and PAR-1-activating peptides, …
An optimized method for intratracheal instillation in mice
NettetBALB/c Mouse Tracheal and Bronchial Epithelial Cells from Creative Bioarray are isolated from tracheal and bronchial tissue of pathogen-free laboratory mice. BALB/c Mouse Tracheal and Bronchial Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s … Nettet1. mar. 2009 · A novel method for endotracheal intubation of mice and rats used in imaging studies. Contemp Top Lab Anim Sci 44: 52–55, 2005. Google Scholar; 9 Spoelstra EN, Ince C, Koeman A, Emons VM, Brouwer LA, van Luyn MJ, Westerink BH, Remie R. A novel and simple method for endotracheal intubation of mice. Lab Anim 41: 128–135, … laundromat for sale toronto gta
Basal cells as stem cells of the mouse trachea and human airway
Nettettrachea and lung tissue and are intended as a guideline, especially for those less familiar with the geropathology of old mice. It is a basic protocol that can be adjusted depending on the experimental needs of the project. Basic protocol 1: Collection of the lungs from old mice Equipment and materials Dissecting board with cork or rubber surface NettetProduct Testing. C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology) antibodies. These cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2-3 passages at a split ratio … Nettet21. des. 2024 · In tracheal cilia of Cfap53-/-mice, distal localization of DNAH9 was maintained, although its level was reduced compared with that apparent in control mice . Unexpectedly, we failed to detect DNAH9 protein in node cilia, either with a DNAH9-specific antibody ( S6 Fig ) or by live imaging of a DNAH9::Venus fusion protein … justin berfield and aaron carter relationship