WebThe Proximity Ligation Assay(PLA) requires specific antibodies directed to the protines of interest. The assay utilizes the Duolink kit as described below. DuolinkR In Situ reagents from OlinkR Bioscience enable … WebIn situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.
Identification and Immunocytochemical Characterization of …
WebDuolink anti-mouse PLUS and MINUS secondary antibodies and the signal amplified with the Red detection system. Cells were counterstained with the DAPI and AlexaFluor 488-phalloidin. Images were taken with a 60x objective. Proximity ligation assay (PLA) is a technology that extends the capabilities of traditional immunoassays to include direct WebTo examine whether indeed ADAM12 and BSG interact at the cell surface, we used the commercially available Duolink ® kit, which is based on the in situ proximity ligation … helsingintie 733 23310 taivassalo
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WebThe Duolink proximity ligation assay was purchased from Sigma-Aldrich and performed in a format suitable for the detection of single proteins and protein-protein interactions, as described in detail previously [30,53,54]. In brief, hVSMC and mouse VSMC were fixed on eight well tissue culture slides (Nunc). WebThe Duolink® PLA signal is recognized as discrete fluorescent spots in various locations of the studied cells (see Figure 1A). Individual signals are of sub-micrometer size and ... Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol, 20, 473-77 (2002). Figure 3. Image analysis using imaging software. The DAPI ... WebProximity ligation assay (PLA) was performed on tissue sections using Duolink PLA In Situ Fluorescence kit (Sigma-Aldrich, Hamburg, Germany) according to the manufacturer’s protocol and using in-house modified diluent and blocking solutions as previously described . In brief, after rehydration, tissues were incubated with 1% BSA in PBS for 1 ... helsingin timanttiporaus apollonia oy